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DOI: 10.21088/ijnns.0975.0223.17125.3

Abstract

Background: Since few decade stem cell research is being more crucial as it has the ability to renew their own population and to differentiate into specialized population of cells. To overcome the immune rejection, ethical issue of embryonic stem cells, and for more practical point of view, patients own body cells be reprogrammed to assume a stem cell like state called induced pluripotent cells (iPSCs) which maintain all the essential properties of embryonic stem cells. Aim: The use of iPS cells for differentiating into various cell type of body and derived differentiate cells to provide personalized cells for cell based therapy is the great aim and it opened the new avenue for regenerative medicine. Objectives: The capability of induced pluripotent stem cells (iPSC) is tremendous as it infinitely divided and differentiated into many different cell types. Hence objective is to use iPSCs to model several diseases to unravel the mechanism of alterations in these diseases. Material: Various approaches were used to induce iPSCs such as delivering reprogramming factors (Oct4, Sox2, Klf4 & c-Myc) into somatic cells or by using a cocktail of small-molecule compounds that modulate various signaling pathways. Result: The tissue of patients are used to generate immune-matched supply of pluripotent stem cells to test or screen the drug and study the specific cause for that disease in patient specific way. The stem cell research including iPS cells has received a significant amount of public attention for neurodegenerative diseases, diabetics and other tissue specific diseases. Conclusion: Great benefits are expected in this field as researchers take advantage of the tremendous potential regenerative properties of induced pluripotent stem cells for promising therapeutic applications, which is described in this article 

Keywords : Background: Since few decade stem cell research is being more crucial as it has the ability to renew their own population and to differentiate into specialized population of cells. To overcome the immune rejection, ethical issue of embryonic stem cells, and for more practical point of view, patients own body cells be reprogrammed to assume a stem cell like state called induced pluripotent cells (iPSCs) which maintain all the essential properties of embryonic stem cells. Aim: The use of iPS cells for differentiating into various cell type of body and derived differentiate cells to provide personalized cells for cell based therapy is the great aim and it opened the new avenue for regenerative medicine. Objectives: The capability of induced pluripotent stem cells (iPSC) is tremendous as it infinitely divided and differentiated into many different cell types. Hence objective is to use iPSCs to model several diseases to unravel the mechanism of alterations in these diseases. Material: Various approaches were used to induce iPSCs such as delivering reprogramming factors (Oct4, Sox2, Klf4 & c-Myc) into somatic cells or by using a cocktail of small-molecule compounds that modulate various signaling pathways. Result: The tissue of patients are used to generate immune-matched supply of pluripotent stem cells to test or screen the drug and study the specific cause for that disease in patient specific way. The stem cell research including iPS cells has received a significant amount of public attention for neurodegenerative diseases, diabetics and other tissue specific diseases. Conclusion: Great benefits are expected in this field as researchers take advantage of the tremendous potential regenerative properties of induced pluripotent stem cells for promising therapeutic applications, which is described in this article

Corresponding Author : Mamata Mishra,