Background: A mass disaster is defined as an event (railway, naval, air, motorway, earthquake, flooding accidents and so on), results in a large number of fatalities (greater than 12 people) that need to be identified. These victims are called as mass disaster victims and the process of identification of these victims is known as disaster victim identification (DVI). Mass disaster can be classified in three type’s vig. Open system, closed system and mixed system. In cases of mass disaster, the scene of disaster can often result in highly inter and intra mixing, decomposition, contamination and fragmentation of the remains of mass disaster victims. The result of traditional identification methods, like anthropological, odontological and X-ray examinations are often difficult to use as they are based on a unique set of physical characteristics. Hence, DNA profiling became a gold standard in disaster victim identification, mass-casualty incidents (MCIs) and any criminal case where the biological sample is highly fragmented or degraded. Aim & Objective: This case study is based on the disaster victim identification (DVI) method using currently available molecular techniques of DNA profiling for the 11 unknown disaster victims/mass-casualty incident victims reported by the police. We identified all 11 dead bodies using five multiplex PCR amplification kits (GlobalFiler™, PowerPlex® Fusion 5C, Investigator Argus X-12 QS System, Yfiler® Plus and PowerPlex® Y23 System PCR Amplification Kit).  Material: In the present case study, 11 semi-charred unknown bone samples and their relative 11 reference sample (blood sample of the suspect on FTA card) were received properly sealed condition with the sample to maintain the integrity and chain of custody of samples at the DNA Fingerprinting division, State Forensic Science Laboratory, Jaipur, Rajasthan for routine casework analysis. Result & Conclusion: On the basis of DNA analysis it is concluded that the source of DNA profile obtained from unknown charred bone sample 1, sample 2, sample 3, sample 4, sample 5, sample 6, sample 7, sample 8, sample 9, sample 10 and sample 11 are biologically related with the source of DNA profile obtained from control blood sample on FTA cards sample 16, sample 19, sample 18, sample 12, sample 17, sample 21, sample 13, sample 15, sample 14, sample 22 and sample 20 respectively.