Background: A number of clinical disorders, particularly those associated with chronic stress, may cause disruptions in cardiac autonomic balance. As a result, parasympathetic tone decreases and sympathetic activity rises. One relaxation method that may help restore this equilibrium is slow breathing pranayama. Aim and Objective: The purpose of this study is to assess how Slow Breathing Pranayama affects cardiac autonomic balance. Materials and Methods: 60 young, healthy, sedentary volunteers participated in a prospective interventional study in which they performed Slow Breathing Pranayama for 12 weeks. Prior to and during the intervention, measurements were made of heart rate (HR), blood pressure (BP), and heart rate variability (HRV). Both time-domain and frequency-domain characteristics were used to measure HRV. The software Kubios HRV (version 1.1, Finland) was used to analyze HRV. Results: HRV measurements showed a change toward parasympathetic predominance, whereas HR and blood pressure showed significant decreases. Conclusion: Practicing slow breathing techniques on a regular basis can help the heart’s autonomic balance move toward parasympathetic dominance and can help prevent illnesses linked to autonomic imbalance.