Pyometra is a life-threatening uterine infection in intact female dogs, commonly caused by Escherichia coli. This report presents a case of open cervix pyometra in a six-year-old intact female Rottweiler, which was presented to the Department of Veterinary Gynaecology and Obstetrics at the College of Veterinary Science, Parbhani, MAFSU, with a 10-day history of anorexia, lethargy, depression, vomiting, polydipsia, polyuria, and sanguinopurulent haemorrhagic vaginal discharge, with no history of mating. Clinical examination revealed dehydration, abdominal distension, leucocytosis (58,000 cells/mm³), neutrophilia, thrombocytopenia (78,000 cells/mm³), and elevated blood urea nitrogen (56.7 mg/dL), indicating systemic involvement. Ultrasonography confirmed a fluidfilled, distended uterus. Based on the history, clinical signs, and ultrasonographic findings, the case was diagnosed as open-cervix pyometra. The dog was stabilized with intravenous fluids, antibiotics (ceftriaxone-tazobactam), and NSAIDs before undergoing ovariohysterectomy under general anaesthesia. The pusfilled uterus was surgically removed, and post-operative management included antibiotics and analgesics. The patient recovered uneventfully, with sutures removed on the 15th postoperative day. This case highlights the importance of early diagnosis, medical stabilization, and surgical intervention in managing pyometra. Ovariohysterectomy remains the gold standard treatment, preventing recurrence and improving survival outcomes.