In offences involving the human body, Laboratory analysis of material obtained from the victim or the accused can prove vital to proper investigation of the case. In case of poisoning, Viscera and Biological Sample are the main physical evidences which play very important role to solve the crime. Unfortunately, Medical Officers are often not aware as to the exact nature or quantity of samples to be taken, nor the correct manner of preservation or packing that should be followed. Collection and preservation of viscera for chemical analysis, it is a duty of doctor to collect and preserve the viscera and other exhibits property. The aim of this literature is to be helpful to medical officers & police personals in Collection  and  Preservation of  Viscera and  Biological Sample in Poisoning cases to solve crime.