Background: Color stability of denture base resins is critical for long-term esthetics and patient satisfaction. Commercial denture cleansers are effective but may affect the color stability of acrylic resins. Herbal alternatives offer potential advantages with fewer side effects. Aim: To evaluate and compare the effect of herbal extracts and commercial denture cleansers on the color stability of heat-polymerized acrylic denture base resin. Materials and Methods: A total of 36 acrylic resin specimens were fabricated and divided into four groups (n=9) based on cleansing solution: Group I – Clinsodent, Group II – Distilled Water, Group III – Oradox, and Group IV – Origanum Oil. All samples were stained with turmeric, tea, and coffee solutions, then subjected to respective cleansing agents for 10 days. Color changes were measured at baseline, post-staining, and post-cleansing using a UV-VIS spectrophotometer and analyzed via CIELAB parameters (?E). Data were statistically analyzed using ANOVA and Tukey’s post hoc test. Results: Origanum oil (Group IV) demonstrated the highest color stability (?E = 77.91 ± 0.13), followed by Oradox (76.01 ± 0.13), Clinsodent (75.35 ± 0.16), and distilled water (72.83 ± 0.20). Statistically significant differences were observed   between all groups (p < 0.001).   Conclusion: Origanum oil exhibited superior performance in maintaining color stability of acrylic resins and can serve as an effective herbal alternative to commercial denture cleansers.