Ganoderma lucidum is a medicinal, facultative parasitic fungus known as reishi mushroom. It produces fruiting body on different living and dead trunks. Different lignocellulosic agricultural wastes are also used as substrate for its artificial cultivation. Among the different factors, supplements play an important role in affecting its productivity. Therefore, five different supplements such as boiled paddy, boiled wheat, wheat bran, neem cake along with control were evaluated. During preparation of bags for G. lucidum, paddy straw was used as substrate along with different supplements. The bags were quadruplicated for each treatment and kept in different tiers following Randomised Block Design (RBD). Among five treatments maximum yield of 28.8g/bag with an average of 3.5 numbers of mushroom obtained from bags supplemented with boiled paddy followed by 27.0g/bag each as in case of boiled wheat and wheat bran. In contrast, neem cake supplementation resulted with the lowest yield, even below that of without any supplements i.e. control. However further study is required to elucidate about the bioactive components of mushroom among different treatments to determine the potential pharmacological variability.