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Journal of Microbiology and Related Research

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A Study of Biofilm Producers and Its Correlation to Antimicrobial Resistance among Orthopaedic Implant Associated Infections in a Tertiary Care Centre

Neeta P.N.**** , Shobha Medegar K.R.* , Pooja Mansabdar** , Asha B. Patil*** , Harshika Y.K.* , Ganesh Perumal** , Neeta P.N.****

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Journal of Microbiology and Related Research 3(1):p 15-19, . | DOI: DOI: http://dx.doi.org/10.21088/jmrr.2395.6623.3117.3
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Abstract

Background: Biofilms are defined as microbially derived sessile communities characterized by the cells that are irreversibly attached to a substratum or to each other. These biofilm forming organisms are frequently associated with implant associated infections and they are intrinsically more resistant to antimicrobial agents than planktonic cells. Objectives: 1. This study aims to detect the biofilm formation in the orthopaedic implant associated infections 2. to correlate its antibiotic susceptibility pattern with emphasis on multi drug resistance pattern. Methodology: A prospective study was done on a total of 150 cases,of aspirated pus sample, of all orthopaedic implant associated infections over a period of one year and sent to the department of microbiology, KIMS, Hubballi, whereinthey were processed according to the standard laboratory protocol. The isolates were identified and subjected to biofilm detection by three methods (Tube method, Tissue culture plate method and Congo red Agar method) and subsequently antibiotic susceptibility testing was performed on Muller Hinton Agar (MHA) by Kirby Bauer’s Disc Diffusion method according to Clinical and Laboratory Standard Institute( CLSI) guidelines 2016. Results: Among 150 samples which were processed for biofilm detection 48% were detected as positive. Majority were Gram Positive Cocci (GPC) accounting to 63.88%. Methicillin Resistant Staphylococcus Aureus (MRSA) accounted to 60%, multi-drug resistance (MDR) was noted in 69.56% in case of GPC and 100% MDR Gram negative bacteria (GNB). onclusion: Biofilm detection methods and its antimicrobial susceptibility testing should be routinely employed especially in case of implant associated infections, so that we can formulate antibiotic regimen for the multi-drug resistant isolates, by appropriate screening of MRSA, Extended Spectrum Beta Lactamase (ESBL), AMP-C and MBL and thus prevent treatment failures. 

Keywords: AMP-C Co-producers; Biofilm; ESBL; Implant; Multi-Drug Resistance and MRSA.

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Authors and Affiliatione

  • Neeta P.N.****
    ,
  • Shobha Medegar K.R.*
    ,
  • Pooja Mansabdar**
    ,
  • Asha B. Patil***
    ,
  • Harshika Y.K.*
    ,
  • Ganesh Perumal**
    ,
  • Neeta P.N.****
    ,
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DOI: DOI: http://dx.doi.org/10.21088/jmrr.2395.6623.3117.3
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