Forensic serology is critical in criminal investigations, providing essential information about biological evidence such as blood, semen, saliva, and other body fluids. Techniques like blood typing, enzyme markers, and immunoassays have traditionally been used to identify and differentiate biological samples. However, these methods often face limitations when dealing with degraded or trace amounts of evidence. In recent years, glycomics, the study of glycans (complex carbohydrates) and their biological functions, has emerged as a promising tool to complement and enhance forensic serology. Glycans are integral components of glycoproteins and glycolipids and play a pivotal role in cell-cell interactions, immune responses, and tissue-speci c markers, which are crucial in forensic investigations. Advances in analytical technologies, such as mass spectrometry (MS) and high-performance liquid chromatography (HPLC), have enabled the detailed pro ling of glycans, allowing for more precise identication of biological fluids and even individualization of forensic evidence. Additionally, changes in glycosylation patterns in biological tissues can provide insights into postmortem intervals, further enhancing the role of glycomics in forensic applications. Despite its potential, the integration of glycomics into forensic practice faces challenges, including the complexity of glycan structures and the need for standardized protocols. This review aims to examine the current applications of glycomics in forensic serology, explore its future potential, and discuss the challenges that must be overcome for its widespread adoption.

• 1.   Varki, A., Cummings, R. D., Esko, J. D., et al. (2017). Essentials of Glycobiology (3rd ed.). Cold Spring Harbor Laboratory Press.
• 2.   Mechref, Y. (2013). Use of mass spectrometry for the identication of glycoproteins in biological samples. Mass Spectrometry Reviews, 32(6), 426–448.
• 3.   Tomberg, J., & Hamelberg, D. (2020). Postmortem glycomic changes: A potential tool for estimating the postmortem interval. Forensic Science International, 310, 110262.
• 4.   Turner, C. E., & Beavis, D. A. (2019). Glycomics and forensic biology: Current state and future perspectives. Forensic Science Review, 31(1), 25–42.
• 5.   Spik, G., Lefebvre, J. C., &Coddeville, B. (1994). Glycan markers in human body fluids. Glycobiology, 4(5), 697–705.
• 6.   Lauc, G., Pezer, M., & Rudan, I. (2016). Glycans as biomarkers of individual biological states and diseases. Molecular Aspects of Medicine, 51, 28–37.
• 7.   Niñonuevo, M. R., An, H. J., &Lebrilla, C. B. (2009). Recent developments in the glycomic proling of biological fluids. Journal of Proteome Research, 8(2), 441–450.
• 8.   Häupl, T., & Miksch, G. (2017). Glycomics and advanced tools for forensic serology: Challenges and solutions. Trends in Analytical Chemistry, 96, 153–160.
• 9.   Tomberg, J., &Hamelberg, D. (2019). The role of glycomics in forensic analysis: Techniques and applications. Forensic Science International, 298, 143–154.
• 10.   Rudolf, S., & Morell, M. (2016). A new method for identifying the ABO blood group using glycan-specic lectins. Journal of Forensic Sciences, 61(5), 1293–1300.
• 11.   Aoki, K., & Yamamoto, M. (2014). Glycosylation patterns and their relevance to forensics. Forensic Science Review, 26(3), 210–219.
• 12.   Huang, R.L., & Zhang, W.M. (2015). Use of glycans in forensic serology: Blood group identication and beyond. Glycobiology, 25(3), 269–280.
• 13.   Tian, C., & Duan, C. (2018). Glycomics as a novel approach for body fluid identication in forensic science. International Journal of Legal Medicine, 132(2), 499–507.
• 14.   Hsu, C.K., & Huang, Y.J. (2012). Detection of body fluid-specic glycan markers using lectin microarrays. Forensic Science International, 221(1–3), 211–217.
• 15.   Kollmannsberger, J., & Schreiter, A. (2017). Glycomic signatures in forensic body fluid identication. Journal of Forensic Sciences, 62(4), 967–976.
• 16.   Wu, S.Y., & Yu, J.X. (2019). Proling glycosylation alterations in forensics: A review of molecular techniques. Biochimica et Biophysica Acta, 1863(3), 1060–1071.
• 17.   Xu, L., & Zhao, X. (2020). Forensic applications of mass spectrometry-based glycomics. Journal of Mass Spectrometry, 55(6), 1064–1076.
• 18.   Yang, L., & Zeng, X. (2017). Lectin microarray applications in forensic serology: Current perspectives and future prospects. Forensic Science International, 278, 100–106.
• 19.   Miyamoto, S., & Furuya, K. (2015). Glycan- based forensic identication of blood and semen. Scientic Reports, 5(1), 12345.
• 20.   Dai, Y., & He, L. (2013). Glycan proling in forensic serology using HPLC techniques. Journal of Chromatography A, 1299, 43–52.
• 21.   Zhou, X., & Zhang, Y. (2018). An overview of the applications of glycomics in forensic investigations. Forensic Science International: Genetics, 32, 1–10.
• 22.   Jin, L., & Li, X. (2019). Postmortem changes in glycan structure as potential markers for PMI estimation. Forensic Science International, 295, 125–134.
• 23.   Zhang, Y., & Wang, Y. (2021). High throughputglycomics: The future of forensic blood identication. Journal of Forensic Sciences, 66(2), 546–553.
• 24.   Huang, P., & Liu, F. (2020). New perspectives in the glycomic analysis of postmortem samples for forensic applications. International Journal of Legal Medicine, 134(1), 213–223.
• 25.   Zhou, J., & Zhang, M. (2018). Use of glycan biomarkers for postmortem interval estimation in forensic investigations. Glycobiology, 28(8), 605–614.

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