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Journal of Forensic Chemistry and Toxicology

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Comparative Analysis of DNA Extraction Techniques and their Analysis from Blood Stained Clothes

Rajesh Kumar, , Vaishali Vardiya1 , Anita Yadav2 , Tanushree Dheer3 , Rajesh Kumar4

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Journal of Forensic Chemistry and Toxicology 10(1):p 21-25, . | DOI: http://dx.doi.org/10.21088/jfct.2454.9363.10124.3
How Cite This Article:

Vaishali Vardiya, Anita Yadav, Tanushree Dheer et al. Comparative Analysis of DNA Extraction Techniques and their Analysis
from Blood Stained Clothes. J Forensic Chemistry Toxicol. 2024;10(1):21–25.
 

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Abstract

The advancement of forensic DNA analysis has revolutionized criminal investigations,  providing crucial evidence for identifying suspects and establishing their involvement in  various crimes. The extraction of DNA from blood stains is a fundamental step in forensic  investigations, enabling the profiling of individuals for identification and genetic analysis.  The objective of this research is to evaluate and compare the DNA extraction methods on different fabric types, which is cotton, and silk. Blood stains were spotted in both the cloths  and incubated for a certain period of time. After which, DNA extraction was done using phenol chloroform method. The quality and quantity of the extracted DNA are assessed using  established techniques such as Gel electrophoresis and qPCR amplification. The results of  the experiments indicate variations in the efficiency of DNA extraction methods depending  on the fabric type. Ideally both the fabrics have shown similar pattern of DNA recovery but  Cotton fabrics demonstrate higher DNA recovery rates, of DNA recovery but Cotton fabrics  demonstrate higher DNA recovery rates, compared to silk fabric but this is non-significant. In conclusion, provides valuable insights into the challenges and opportunities associated with  DNA extraction from blood stains on different fabrics for forensic investigation profiling. The  research findings contribute to the advancement of forensic DNA analysis and offer practical  guidance for forensic scientists to optimize their methods when handling blood-stained fabric  evidence. Further research and development in this field will undoubtedly strengthen the   forensic community’s ability to solve crimes and administer justice with greater accuracy and  reliability  

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  • Rajesh Kumar,
    ,
  • Vaishali Vardiya1
    ,
  • Anita Yadav2
    ,
  • Tanushree Dheer3
    ,
  • Rajesh Kumar4
    ,
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Cite this article

Vaishali Vardiya, Anita Yadav, Tanushree Dheer et al. Comparative Analysis of DNA Extraction Techniques and their Analysis
from Blood Stained Clothes. J Forensic Chemistry Toxicol. 2024;10(1):21–25.
 

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DOI: http://dx.doi.org/10.21088/jfct.2454.9363.10124.3
Keywords DNA Extraction; Human DNA; Forensic DNA.

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