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Isolation and Characterization of Pseudomonas Aeruginosa Bacteriophages

Chand Pasha, Assistant Professor, Department of Microbiology, Nizam College, Osmania University, Hyderabad 500001, Telangana, India. , Jangili Pavan Kumar1 , Shaik Muzammil Pasha2 , Yemgdda Goutham Sudhan3 , Chand Pasha4

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Journal of Microbiology and Related Research 9(1):p 9-16, . | DOI: http://dx.doi.org/10.21088/jmrr.2395.6623.9123.1
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Jangili Pavan Kumar, Shaik Muzammil Pasha, Yemgdda Goutham Sudhan et al./Isolation and Characterization of Pseudomonas
Aeruginosa Bacteriophages/J Microbiol Relat Res. 2023;9(1): 09–16.

Received : N/A         Accepted : N/A          Published : N/A
Abstract

Pseudomonas aeruginosa is a notorious, opportunistic pathogen and has ability of biofilm formation making it possible to survive in extreme conditions. As antibiotics and disinfectants have shown limited activity, P. aeruginosa phage based treatments are proposed as a promising way for biofilm control. P. aeruginosa isolates and PB1 like phage particles were isolated from sewage samples collected from sewage treatment plants. PB1 like phage was featured with large burst size of 151PFU/cell, host inactivation in 6 hrs, 80% of host range and had icosahedral head with a diameter of 85X80nm and long non contractile tail of 135nm. PB1 like phages are suitable for lysis of broad range MDR Pseudomonas biofilms, which could be used in phage therapy.

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Authors and Affiliatione

  • Chand Pasha, Assistant Professor, Department of Microbiology, Nizam College, Osmania University, Hyderabad 500001, Telangana, India.
    ,
  • Jangili Pavan Kumar1
    ,
  • Shaik Muzammil Pasha2
    ,
  • Yemgdda Goutham Sudhan3
    ,
  • Chand Pasha4
    ,
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Jangili Pavan Kumar, Shaik Muzammil Pasha, Yemgdda Goutham Sudhan et al./Isolation and Characterization of Pseudomonas
Aeruginosa Bacteriophages/J Microbiol Relat Res. 2023;9(1): 09–16.

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DOI: http://dx.doi.org/10.21088/jmrr.2395.6623.9123.1
Keywords Pseudomonas aeruginosa; Bacteriophages; PB1; Cell lysis; Biofilm.

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