" "
Home > Journals > Journal of Microbiology and Related Research

Journal of Microbiology and Related Research

Submit your article Read this Journals View all journals
Full Text (PDF)
Original Article

Isolation of MTB Strains and Determining the Antibiotic Susceptibility Pattern via Bactec 320 from the Females of Child Bearing Age

D. Bisht** , P.S. Gangania* , D. Bisht** , V.A. Singh***

Author Information
Licence:


Journal of Microbiology and Related Research 3(1):p 43-48, . | DOI: DOI: http://dx.doi.org/10.21088/jmrr.2395.6623.3117.7
How Cite This Article:
Received : N/A         Accepted : N/A          Published : N/A
Abstract

 Background: The BACTEC MGIT 320 is a new, lower-capacity instrument for liquid culture developed for the growth and detection of M. tuberculosis and antimicrobial susceptibility testing. MGIT has an improved speed and sensitivity of MTB isolation and drug susceptibility testing, irrespective of the HIV status of the patient. This study was undertaken to find the appropriate antibiotic susceptibility pattern of confirmed positive MTB strains via MGIT 320 liquid culture technique. Objectives: Isolation of positive MTB strains and determination of their antibiotic susceptibility pattern using BACTEC 320 from the females of child bearing age group. Material and Methods: A total of 217 samples were processed involving the isolation of MTB strains along with the antibiotic susceptibility pattern of positive MTB’s. Both techniques were used culture and RT-PCR to find the prevalence and AST via BACTEC 320 system. Analysis of the results was done at the end of the procedure. Results: Out of 217 samples, there were 29 positive MTB strains by RTPCR technique whereas via liquid culture there were 11 positive MTB strains. The Antibiotic susceptibility pattern for MTB positive strains for both the first and second line was Levofloxacin, Kanamycin and PAS were found to be much sensitive whereas Isoniazid and Ethionamide were found to be more resistant than others. Conclusion: RT-PCR technique detects the total count of mycobacterial bacilli whereas via liquid culture only viable bacteria are detected i.e. true positive MTB strains. There were 2 MDR and 2 XDR TB detected, out of 11 confirmed MTB strains.

Keywords: AST; BACTEC 320; MTB Strains; RT-PCR Technique; Liquid Culture.

References

No records found.

Funding

Author Information

Authors and Affiliatione

  • D. Bisht**
    ,
  • P.S. Gangania*
    ,
  • D. Bisht**
    ,
  • V.A. Singh***
    ,
Conflicts of Interest
Supplementary Information

Below is the link to the supplementary material.

Rights and Permissions


About this article

Cite this article

Licence:


Download citation

Received Accepted Published
N/A N/A N/A
DOI: DOI: http://dx.doi.org/10.21088/jmrr.2395.6623.3117.7
Keywords

Article Level Metrics

Last Updated

Sunday 08 June 2025, 05:50:07 (IST)

6

Accesses

00
0
00

Citations


22
11
23

View full article metrics including social shares, article views and publishing history


Article Keywords

Keyword Highlighting

Highlight selected keywords in the article text.


Timeline

Received N/A
Accepted N/A
Published N/A

licence




Access this article


Reprint Order Form
Buy Single Article
Institutional Subscriptions

Share