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Method Validation and Quantitative Estimation of Ethanol using n-Propanol as Internal Standard in whole Blood by Gas Chromatography - Headspace (GC-HS)

Ashok Kumar Jaiswal , Ashok Kumar Jaiswal 1 , Supriya Krishna 2 , Khoob Chand 3 , Tabin Millo 4 , Sudhir Kumar Gupta 5

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Journal of Forensic Chemistry and Toxicology 5(1):p 5-9, . | DOI: http://dx.doi.org/10.21088/jfct.2454.9363.5119.1
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Ashok Kumar Jaiswal, Supriya Krishna, Khoob Chand et al. Method Validation and Quantitative Estimation of Ethanol using n-Propanol as Internal Standard in whole Blood by Gas Chromatography - Headspace (GC-HS). J Forensic Chemistry Toxicol. 2019;5(1):5-9.

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Abstract

Detection and quantification of ethanol in drunken driving cases is of immense importance inforensic toxicology. Despite several analytical methods being available for identification of ethanol in blood, an accurate quantification with minimum sample preparation and rapid analysis is still an ongoing task. The present study evaluates the suitability of Headspace-Chromatography with a capillary column and Flame Ionisation Detector as a method for determining the ethanol content in whole blood samples received for the blood alcohol concentration analysis. An internal standard ‘n-propanol’ was added to the sample to authenticate the results. The peaks areas were measured and calculations were carried out considering peak ratios of analyte to internal standard. The total run time of GC and HS was 20.68 min. The validation study for the method resulted in linearity for a range of 7.9 mg- 237 mg/100 ml, coefficient of variance (R2) was 0.999. Recovery of more than 96% was achieved in spiked samples. LOD and LOQ were 0.20 mg/100 ml and 1.0 mg/100 ml respectively. Blood ethanol measurement by this method is an easy, simple, reliable and reproducible.

 

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  • Ashok Kumar Jaiswal
    ,
  • Ashok Kumar Jaiswal 1
    ,
  • Supriya Krishna 2
    ,
  • Khoob Chand 3
    ,
  • Tabin Millo 4
    ,
  • Sudhir Kumar Gupta 5
    ,
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Ashok Kumar Jaiswal, Supriya Krishna, Khoob Chand et al. Method Validation and Quantitative Estimation of Ethanol using n-Propanol as Internal Standard in whole Blood by Gas Chromatography - Headspace (GC-HS). J Forensic Chemistry Toxicol. 2019;5(1):5-9.

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DOI: http://dx.doi.org/10.21088/jfct.2454.9363.5119.1
Keywords GC-HS; Alcohol; ICH Guidelines; FID; Whole Blood etc.

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