Home > Journals > Indian Journal of Forensic Medicine and Pathology

Indian Journal of Forensic Medicine and Pathology

Submit your article Read this Journals View all journals
Full Text (PDF)
Original Article

Role of Oxidative Stress Associated Molecular Diagnostic Signatures in the Personal Identification of Rheumatoid Arthritis Patients

Rahul Saxena , 1Rahul Saxena , 2 Ankit Batra , 3Rajni Ranjan , 4Jyoti Batra , 5Mausami Bhardwaj , 6 Merajul Haque Siddiqui

Author Information
Licence:


Indian Journal of Forensic Medicine and Pathology 14(3(Special Issue)):p 555-565, . | DOI: DOI: http://dx.doi.org/10.21088/ijfmp.0974.3383.14321.18
How Cite This Article:

How to cite this article, Rahul Saxena. Role of oxidative stress associated molecular diagnostic signatures in the personal identification of rheumatoid arthritis patients. Indian J Forensic Med Pathol. 2021;14(3 Special):555-565

Received : N/A         Accepted : N/A          Published : N/A
Abstract

ABSTRACT
context: Early interpretation of the toxic effect of free radicals leading to cardiovascular diseases (CVDs) in autoimmune disorders is a quenching thirst for therapeutic intervention and personal identification. aim: The study aims to evaluate the expression of Nrf2, NQO-1, and LpPLA2 genes along with markers of endothelial dysfunction and oxi-inflammatory stress in active RA patients and to enlighten the assessment of study group markers in the personal identification of RA patients for their early therapeutic intervention and prevention of vascular complications. materials & method: 64 active RA patients between 35-55 years and 64 healthy controls were recruited from North India region. Using specific primers, mRNA expression of Nrf2, NQO-1 & LpPLA2 genes were evaluated in blood by qPCR. 2 –ΔΔCT method was used to determine the fold change. Brachial artery flow mediated diameter (FMD), total antioxidant activity, malondialdehyde, TNF-α, IL-6 & hs-CRP levels were estimated by using standard methods followed by appropriate statistical analysis of data. results: Increased expression of NQO-1, Nrf2 and LpPLA2 was observed in RA patients along with marked altered levels (p<0.05; significant) of oxiinflammatory markers which may be due to compensatory activation of antioxidant defense mechanism. Remarkably, FMD% was significantly low (p<0.05) and inversely associated with the expression of NQO-1, Nrf2 and LpPLA2, which highlighted the culprit effect of oxi-inflammatory stress in inducing altered vascular homeostasis. conclusions: Thus, combinational analysis of molecular diagnostic signatures associated with toxic free radicals along with FMD measurement exhibits a great promise in personal identification of RA patients for early therapeutic intervention, mainly, by targeting the oxi-inflammatory stress mediated cytoprotective pathway, and thereby, reducing the burden of CVD morbidity and mortality in RA patients.

References

No records found.

Funding

Author Information

Authors and Affiliatione

  • Rahul Saxena
    ,
  • 1Rahul Saxena
    ,
  • 2 Ankit Batra
    ,
  • 3Rajni Ranjan
    ,
  • 4Jyoti Batra
    ,
  • 5Mausami Bhardwaj
    ,
  • 6 Merajul Haque Siddiqui
    ,
Conflicts of Interest
Supplementary Information

Below is the link to the supplementary material.

Rights and Permissions


About this article

Cite this article

How to cite this article, Rahul Saxena. Role of oxidative stress associated molecular diagnostic signatures in the personal identification of rheumatoid arthritis patients. Indian J Forensic Med Pathol. 2021;14(3 Special):555-565

Licence:


Download citation

Received Accepted Published
N/A N/A N/A
DOI: DOI: http://dx.doi.org/10.21088/ijfmp.0974.3383.14321.18
Keywords

Article Level Metrics

Last Updated

Sunday 08 June 2025, 02:31:07 (IST)

60

Accesses

00
0
00

Citations


22
11
23

View full article metrics including social shares, article views and publishing history


Article Keywords

Keyword Highlighting

Highlight selected keywords in the article text.


Timeline

Received N/A
Accepted N/A
Published N/A

licence




Access this article


Reprint Order Form
Buy Single Article
Institutional Subscriptions

Share