" "
Home > Journals > Journal of Forensic Chemistry and Toxicology

Journal of Forensic Chemistry and Toxicology

Submit your article Read this Journals View all journals
Full Text (PDF)
Original Article

Seperation and Detection of Nitrazepam and Clonazepam by Thin-Layer Chromatography

Mali Bhagwat D. , Mali Bhagwat D. , Sathe Ravindra A. , Vaishnav Govind R.

Author Information
Licence:


Journal of Forensic Chemistry and Toxicology 1(1):p 17-20, . | DOI:
How Cite This Article:
Received : N/A         Accepted : N/A          Published : N/A
Abstract

 The benzodiazepines are most frequently encountered drugs in emergency toxicology screening, drugs of abuse testing and forensic medicine examinations. The identification of nitrazepam and clonazepam, the most frequently used benzodiazepines is described by simple, rapid and sensitive thin­layer chromatographic method. The structures of these benzodiazepines contain nitro group which is reduced by acidified stannous chloride to give amino group. This couples with diazotized sulphanilic acid to give a yellow brown coloured compound. The detection limit was found to be 3µg and 5µ per spot for nitrazepam and clonazepam respectively. The reaction was not given by other benzodiazepines commonly encountered in toxicological screening.

Keywords: Benzodiazepines; Thin­Layer Chromatography; Spray Reagent; Stannous Chloride; Sulphanilic Acid. 

References

No records found.

Funding

Author Information

Authors and Affiliatione

  • Mali Bhagwat D.
    ,
  • Mali Bhagwat D.
    ,
  • Sathe Ravindra A.
    ,
  • Vaishnav Govind R.
    ,
Conflicts of Interest
Supplementary Information

Below is the link to the supplementary material.

Rights and Permissions


About this article

Cite this article

Licence:


Download citation

Received Accepted Published
N/A N/A N/A
DOI:
Keywords

Article Level Metrics

Last Updated

Sunday 08 June 2025, 09:30:13 (IST)

3

Accesses

00
0
00

Citations


22
11
23

View full article metrics including social shares, article views and publishing history


Article Keywords

Keyword Highlighting

Highlight selected keywords in the article text.


Timeline

Received N/A
Accepted N/A
Published N/A

licence




Access this article


Reprint Order Form
Buy Single Article
Institutional Subscriptions

Share