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Journal of Microbiology and Related Research

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Study of Aerobic Isolates from Vaginal Swabs in Pregnant Women in a Stand-alone Diagnostic Laboratory of Central Madhya Pradesh

Sodani Sadhna , Sodani Sadhna , Hawaldar Ranjana

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Journal of Microbiology and Related Research 1(2):p 77-81, . | DOI:
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Abstract

 Introduction: Colonisation of vagina and cervical epithelium by a variety of micro organisms can cause pelvic infection in pregnant women. The pH and enzymes present in the vaginal secretions determines the type of micro organisms which may cause infection. The knowledge of the commensal bacterial flora of vagina and cervical epithelium is important in pregnancy as many complications may arise due to infection like premature rupture of membranes, puerperal fever, intra or post partum neonatal infections via upper respiratory tract or the umbilical cord Bacterial vaginosis (BV) occurs in about 20 % of the pregnant women and has been associated with adverse pregnancy out comes. The present retrospective study was undertaken with the primary aim of analyzing the common micro organisms causing vaginal infections in pregnant women coming to our microbiology department for vaginal swab cultures. Materials and Methods: High vaginal swabs were collected from 267 pregnant women coming to our Microbiology Department with vaginal symptoms from June 2014 to May 2015. Samples were collected with sterile swabs and immediately inoculated on blood and MacConkey agar plates. After 24 hours, Gram’s stain of pure colonies was done to indentify the organism and accordingly panel was selected for identification and sensitivity on Vitec II (Biomerieux). Results: A total of 159 / 267 (59.5 %) cultures were sterile. 108 / 267 (40.5 %) cultures showed growth of micro organisms out of which E.coli was isolated in 22 (20.3 %), Candida (33.3 %), Staph. aureus (20.3 %), group B Streptococci (11.1 %) and Klebsiella (2.7 %). Candida species was the commonest organism isolated, followed by Staph aureus and E.coli, group B Streptococci and rarely Klebsiella . onclusion: Lactobacilli constitute the major vaginal flora in women during child bearing age and is helpful in maintaining the acidic pH of vagina so that organisms do not invade and cause disease. The natural history of abnormal vaginal flora in pregnancy is still poorly understood but that it can cause perinatal morbidity and mortality is a well known fact. So, for a healthy perinatal outcome of pregnancy, a vaginal swab culture is required in all pregnant women.  

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  • Sodani Sadhna
    ,
  • Sodani Sadhna
    ,
  • Hawaldar Ranjana
    ,
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