" "
Home > Journals > Journal of Microbiology and Related Research

Journal of Microbiology and Related Research

Submit your article Read this Journals View all journals
Full Text (PDF)
Original Article

To Characterize & Determine the Virulence Factors of Staphylococcus aureus in a Tertiary Care Hospital

Dalip K Kakru, Professor & Head, Department of Microbiology, Sher-i-Kashmir Institute of Medical Sciences (SKIMS), Srinagar, Jammu and Kashmir 190011, India. , Dalip K. Kakru* , Mohd Suhail Lone** , Bakhshi Fariyal*** , Junaid Ahmad****

Author Information
Licence:


Journal of Microbiology and Related Research 3(2):p 107-112, . | DOI: http://dx.doi.org/10.21088/jmrr.2395.6623.3217.10
How Cite This Article:
Received : N/A         Accepted : N/A          Published : N/A
Abstract

Context: Staphylococcus is a gram-positive, non-motile, nonsporing bacteria that include different opportunistic pathogenic species, responsible for human and animal infections. Aims: To isolate and identify Staphylococcus aureus from different human clinical samples and to determine various virulence factors of these isolates. Settings and Design: Prospective study conducted in Dept of Microbiology, SKIMS J&K. Methods and Material: Various clinical samples eg pus, blood, sputum etc received in the Microbiology laboratory were processed for isolation of Gram positive cocci. Identification of Staphylococcus aureus was done by standard microbiological methods and various virulence factors were determined. Statistical Analysis Used: Descriptive statistics (frequency and percentage) was used. Results: A total of 217 strains of Staphylococcus aureus were isolated from 1100 clinical specimens. All the isolates had ability to produce free and bound coagulase enzyme. Out of 217 strains, 42 (19.35%)strains showed only  hemolysin production, 84 (38.70%) strains showed only  hemolysin production and rest 91(41.93%) strains showed both  and hemolysin production. Among all the strains, 203 (93.54%) were positive for producing biofilm and rest of 13 were negative for producing biofilm. Out of 217 strains of S.aureus only 49 (22.58%) strains were able to produce the staphyloxanthin pigment and rest 168 (77.41%) were found to be negative. All strains of S.aureus were sensitive to Linezolid, Teicoplanin and vancomycin. On the other hand strains showed high resistance towards Cefoxitin (65.89%). Conclusions: Prevalence of methicillin rsistance was high in IPD setting (65.89%) and production of various virulence factors like coagulase, hemolysin production, biofilm production and staphyloxanthin pigment production was more common in MRSA than in MSSA thus suggesting that infection control policies should be adhered positively.

Keywords: Staphylococcus Aureus; Prevelance; Virulence Factors. 

References

No records found.

Funding

Author Information

Authors and Affiliatione

  • Dalip K Kakru, Professor & Head, Department of Microbiology, Sher-i-Kashmir Institute of Medical Sciences (SKIMS), Srinagar, Jammu and Kashmir 190011, India.
    ,
  • Dalip K. Kakru*
    ,
  • Mohd Suhail Lone**
    ,
  • Bakhshi Fariyal***
    ,
  • Junaid Ahmad****
    ,
Conflicts of Interest
Supplementary Information

Below is the link to the supplementary material.

Rights and Permissions


About this article

Cite this article

Licence:


Download citation

Received Accepted Published
N/A N/A N/A
DOI: http://dx.doi.org/10.21088/jmrr.2395.6623.3217.10
Keywords

Article Level Metrics

Last Updated

Sunday 08 June 2025, 04:08:24 (IST)

6

Accesses

00
0
00

Citations


22
11
23

View full article metrics including social shares, article views and publishing history


Article Keywords

Keyword Highlighting

Highlight selected keywords in the article text.


Timeline

Received N/A
Accepted N/A
Published N/A

licence




Access this article


Reprint Order Form
Buy Single Article
Institutional Subscriptions

Share