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Cytological Assessment of Barr Bodies in Buccal Mucosal Smears and Peripheral Smear in Sex Determination

Karra Shilpa, Associate Professor, Department of Pathology, Mysore Medical College & Research Institute, Mysuru–570001, Karnataka, India. , Karra Shilpa1 , Gayathri M.N.2 , Monica G.S.3

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Indian Journal of Forensic Medicine and Pathology 10(3):p 196-199, . | DOI: http://dx.doi.org/10.21088/ijfmp.0974.3383.10317.4
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Abstract

Introduction: Barr body has been recognized as the cytological manifestation of the inactive X chromosome in interphase nuclei in a highly condensed state. Aims and Objectives: To assess the efficacy of sex determination between pap stained buccal mucosal scrapes and leishman stained drumstick appearance in peripheral smear. Material and Methods: Buccal mucosal smears and peripheral smears are collected from 100 individuals who visit K. R. hospital and Cheluvamba hospital of age ranging from 20-30 years. The buccal mucosal scrapes were stained with papanicolaou stain and peripheral smear of the same individual were stained with leishman stain. Results: In females and males drumstick appearance was more compared to barr body. Conclusion: Sex determination using buccal scrapes and peripheral smear stained with papanicolaou stain and leishman stain proved to be simple, accurate and cost effective method in our study.

Keywords: Barr Body; Drumstick; Papanicolaou Stain; Leishman Stain. 

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  • Karra Shilpa, Associate Professor, Department of Pathology, Mysore Medical College & Research Institute, Mysuru–570001, Karnataka, India.
    ,
  • Karra Shilpa1
    ,
  • Gayathri M.N.2
    ,
  • Monica G.S.3
    ,
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DOI: http://dx.doi.org/10.21088/ijfmp.0974.3383.10317.4
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